Ultrasensitive Insulin ELISA
Ultrasensitive Insulin ELISA
(10-1132-01)
Kit format: 1×96 wells
Measurement range: 0.15 - 20 mU/L (0.0065 - 0.87 μg/L)
1.Calibrated against 1st International Reference Preparation 66/304 for human insulin
2.No cross-reactivity to C-peptide or Proinsulin des(31-32) or split (32-33)
3.Insignificant or no cross-reactivity to insulin analogs
4.CE/IVD labeled on select markets
5.Controls available: 10-1134-01 (US only) or 10-1164-01
DESCRIPTION
A high quality enzyme immunoassay for specific quantification of human insulin in serum or plasma.
The Mercodia insulin assays have little or no cross-reactivity to insulin analogs, rat or mouse insulin, which enables distinguishing endogenous human insulin, both in patients undergoing treatment with insulin analogs, and in rat or mouse models.
The Mercodia Insulin ELISAs are the only ELISA assays to satisfy the total error requirements in the Insulin Standardization Program.
TEST CHARACTERISTICS
SPECIES：Human
SAMPLES：Serum, plasma
SAMPLE VOLUME：25μL
ASSAY RANGE：0.15 - 20 mU/L (0.0065 - 0.87 μg/L)
DETECTION LIMIT：≤ 0.15 mU/L (0.0065 μg/L)
INCUBATION：1h + 30 min
DETECTION METHOD：Absorbance
SPECIFICITY
The following cross-reactions have been tested:
C-PEPTIDE：<0.01 %
PROINSULIN：<0.01 %
PROINSULIN DES (31-32)：<0.5 %
PROINSULIN SPLIT (32-33)：<0.5 %
PROINSULIN DES (64-65)：98 %
PROINSULIN SPLIT (65-66)：56 %
INSULIN ASPART：3 %
INSULIN DETEMIR：Not detected
INSULIN GLARGIN：12%
INSULIN GLULISINE：Not detected
INSULIN LISPRO：Not detected
IGF-I：<0.02 %
IGF-II：<0.02 %
RAT INSULIN：0.7 %
MOUSE INSULIN：0.3 %
PORCINE INSULIN：93 %
OVINE INSULIN：48 %
BOVINE INSULIN：31 %
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