Glucagon ELISA -10μL
Glucagon ELISA – 10 Microliter
(10-1281-01)
Kit format: 1×96 wells
Measurement range: 2-180 pmol/L (7 - 627 pg/mL)
1.Low sample volume - 10 μL
2.No purification of samples
3.Highly appreciated and often cited assay
4.Controls available: 10-1286-01
5.Little or no cross-reactivity to oxyntomodulin, glicentin, mini-glucagon, GLP-1, GLP-2 and GRPP
DESCRIPTION
Mercodia Glucagon ELISA – 10 μL is a highly specific and sensitive immunoassay for measuring glucagon in serum, EDTA plasma, and cell culture media samples from rat, mouse, and non-human primates.
A revolutionizing ELISA for sensitive and specific determination of glucagon in rat, mouse, and non-human primate (NHP) samples. A research tool advancement that allows science to take the next step.
The highly specific monoclonal antibodies used in this assay enable accurate determination of glucagon with low to no levels of significant cross-reactivity to other circulating pro-glucagon-derived peptides. Under certain conditions like post-bariatric surgery, the concentrations of glicentin have been demonstrated to be elevated to levels that might interfere.
TEST CHARACTERISTICS
SPECIES：Rat, mouse and non-human primates (NHP)
SAMPLES：Serum, EDTA plasma and cell culture medium
SAMPLE VOLUME：10μL
ASSAY RANGE：2-180 pmol/L (7 - 627 pg/mL)
DETECTION LIMIT：≤1.5 pmol/L
INCUBATION TIME：18-22 h (overnight) + 15 min
DETECTION METHOD：Absorbance
SPECIFICITY
The following cross-reactions have been tested:
MINI-GLUCAGON：Not detected
GLICENTIN, HUMAN：1.0 %
GLICENTIN, MOUSE：7.0 %
GLICENTIN, RAT：4.0 %
GLP-1 (7-36)：Not detected
GLP-1 (9-36)：Not detected
GLP-2：Not detected
OXYNTOMODULIN, HUMAN/RAT/MOUSE：2.0 %
OXYNTOMODULIN, BOVINE/CANINE/PORCINE：Not detected
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